Capillary electrophoresis mass spectrometry-based untargeted metabolomics to approach disease diagnosis

Metabolites are the final products of metabolism and are, therefore, directly related to phenotype. They constitute metabolome an organism. The wide diversity physicochemical properties metabolites in terms molecular weight, concentration, polarity, volatility, solubility, pKa, charge makes their analysis a remarkable challenge. With over 220,000 recorded HMDB database, there is no single analytical technique capable analyzing all them. Therefore, multiple platforms required obtain comprehensive picture metabolome. Among these platforms, mass spectrometry (MS)-based techniques among most widely used. Capillary electrophoresis (CE) coupled MS has been employed analyze polar/ionic metabolites. Although this not used, it demonstrated unique capabilities for detection polar ionic that essential part usually detected by other techniques. This review highlights role CE-MS untargeted metabolomics, particularly comparison hydrophilic interaction chromatography (HILIC) separation mode. Additionally, we discuss metabolomics workflow including sample treatment analysis, data treatment, metabolite annotation. We notably present annotation tools developed explicitly CE-MS, as well some computational alternatives, in-house libraries relative migration times, effective mobility, MS/MS fragmentation, in-source CEU Mass Mediator online tool. Finally, mention future perspectives technique, such cell-CE ion mobility (IM)-MS. Overall, shows important studies published last five years approach human diseases.